CRISPR文庫是基于CRISPR/Cas9技術建立的高通量基因篩選方案,研究基因在代謝通路或某些特定疾病發生過程中的功能,確認與表型相關的基因或篩選藥物新靶點。

泓迅科技專注于CRISPR基因編輯技術的研究與開發,在CRISPR定制化文庫構建服務的基礎上,為持續提升產品和服務供應能力,從基因、細胞、免疫系統等不同研究層面推出100多種Human pathway sgRNA文庫,為特定通路的靶向篩選等相關研究提供高效工具。

產品優勢

  1. 100+ Human pathway sgRNA文庫供客戶選擇
  2. 文庫的靶點基因是通過KEGG與較新的NCBI RefSeq人基因組數據交叉、并與科學研究前沿相結合下的選擇
  3. 泓迅科技SynoGPS平臺下的獨家算法設計,每個靶點包含4條低脫靶效率的sgRNA序列
  4. 200+定制化文庫交付經驗,保證產品質量
  5. 相關客戶陸續發表文獻26篇,最高影響因子達36.558

人源Pathway sgRNA文庫信息表如下:

代謝過程 文庫編號 通路名稱 載體 基因數目
常見通路(現貨) SHPF231311 ABC transporter pathway Lentisyn005 100
SHPF231312 Acute myeloid leukemia pathway 45
SHPF231313 Adipogenesis pathway 126
SHPF231314 Adult stem cell pathway 58
SHPF231315 Angiogenesis pathway 92
SHPF231316 Apoptosis pathway 68
SHPF231317 cAMP&Ca pathway 91
SHPF231318 Cell cycle pathway 84
SHPF231319 Chemokine pathway 134
SHPF231320 Drug Resistance pathway 348
SHPF231321 ES differentiation pathway 334
SHPF231322 GPCR pathway 280
SHPF231323 Hormone activity pathway 108
SHPF231324 Ion channel pathway 67
SHPF231325 P450 pathway 57
SHPF231326 Tumor metastasis pathway 60
SHPF231327 Tumor suppressor pathway 720
SHPF231328 Neelamegham human glycogene pathway 347
SHPF231329 Human mTORC1 pathway 713
SHPF231330 Pan druggable library 75
SHPF231331 Human interferon stimulated gene pathway 1914
DNA復制與修復 SHPF231001 Base excision repair 33
SHPF231002 DNA replication 36
SHPF231003 Fanconi anemia pathway 54
SHPF231004 Homologous recombination 41
SHPF231005 Mismatch repair 23
SHPF231006 Non-homologous end-joining 13
SHPF231007 Nucleotide excision repair 47
轉錄 SHPF232001 Basal transcription factors 45
SHPF232002 RNA polymerase 34
SHPF232003 Spliceosome 154
翻譯 SHPF233001 Aminoacyl-tRNA biosynthesis 66
SHPF233002 mRNA surveillance pathway 97
SHPF233003 Nucleocytoplasmic pathway 108
SHPF233004 Ribosome biogenesis in eukaryotes 118
SHPF233005 Ribosome 167
運輸與分解代謝 SHPF234001 Autophagy 141
SHPF234002 Endocytosis 251
SHPF234003 Lysosome 132
SHPF234004 Mitophagy 72
SHPF234005 Peroxisome 82
SHPF234006 Phagosome 152
細胞群落 SHPF235001 Adherens junction 71
SHPF235002 Focal adhesion 201
SHPF235003 Gap junction 88
SHPF235004 Signaling pathways regulating pluripotency of stem cells 143
SHPF235005 Tight junction 169
細胞死亡 SHPF236001 Apoptosis 136
SHPF236002 Cell cycle 127
SHPF236003 Cellular senescence 156
SHPF236004 Ferroptosis 41
SHPF236005 Necroptosis 159
SHPF236006 Oocyte meiosis 131
SHPF236007 p53 signaling pathway 73
細胞運動 SHPF236101 Regulation of actin cytoskeleton 229
折疊,分選及降解 SHPF237001 Proteasome 46
SHPF237002 Protein export 23
SHPF237003 Protein processing in endoplasmic reticulum 171
SHPF237004 RNA degradation 79
SHPF237005 SNARE interactions in vesicular transport 33
SHPF237006 Sulfur relay system 8
SHPF237007 Ubiquitin mediated proteolysis 142
生長與再生 SHPF238001 Axon guidance 182
SHPF238002 Osteoclast differentiation 128
信號轉導 SHPF239001 AMPK signaling pathway 121
SHPF239002 Apelin signaling pathway 139
SHPF239003 Calcium signaling pathway 240
SHPF239004 cAMP signaling pathway 225
SHPF239005 cGMP-PKG signaling pathway 167
SHPF239006 ErbB signaling pathway 85
SHPF239007 FoxO signaling pathway 131
SHPF239008 Hedgehog signaling pathway 56
SHPF239009 HIF-1 signaling pathway 109
SHPF239010 Hippo signaling pathway 157
SHPF239011 JAK-STAT signaling pathway 166
SHPF239012 MAPK signaling pathway 294
SHPF239013 mTOR signaling pathway 156
SHPF239014 NF-kappa B signaling pathway 104
SHPF239015 Notch signaling pathway 59
SHPF239016 Phosphatidylinositol signaling system 97
SHPF239017 Phospholipase D signaling pathway 148
SHPF239018 PI3K-Akt signaling pathway 354
SHPF239019 Rap1 signaling pathway 210
SHPF239020 Ras signaling pathway 236
SHPF239021 Sphingolipid signaling pathway 119
SHPF239022 TGF-beta signaling pathway 95
SHPF239023 TNF signaling pathway 114
SHPF239024 VEGF signaling pathway 59
SHPF239025 Wnt signaling pathway 170
信號分子與互作 SHPF231011 Cell adhesion molecules 157
SHPF231012 Cytokine-cytokine receptor interaction 295
SHPF231013 ECM-receptor interaction 88
SHPF231014 Neuroactive ligand-receptor interaction 367
SHPF231015 Viral protein interaction with cytokine and cytokine receptor 100
循環系統 SHPF231111 Adrenergic signaling in cardiomyocytes 154
SHPF231112 Cardiac muscle contraction 87
SHPF231113 Vascular smooth muscle contraction 134
免疫系統 SHPF231211 Antigen processing and presentation 78
SHPF231212 B cell receptor signaling pathway 82
SHPF231213 Chenokine signaling pathway 192
SHPF231214 Complement and coagulation cascades 86
SHPF231215 C-type lectin receptor signaling pathway 104
SHPF231216 Cytosolic DNA-sensing pathway 66
SHPF231217 Fc epsilon RI signaling pathway 68
SHPF231218 Fc ganna R-mediated phagocytosis 97
SHPF231219 Hematopoietic cell lineage 99
SHPF231220 IL-17 signaling pathway 94
SHPF231221 Intestinal immune network for IgA production 49
SHPF231222 Leukocyte transendothelial migration 114
SHPF231223 Natural killer cell mediated cytotoxicity 132
SHPF231224 Neutrophil extracellular trap formation 190
SHPF231225 NOD-like receptor signaling pathway 186
SHPF231226 Platelet activation 124
SHPF231227 RIG-I-like receptor signaling pathway 71
SHPF231228 T cell receptor signaling pathway 104
SHPF231229 Th1 and Th2 cell differntiation 92
SHPF231230 Th17 cell differentiation 108
SHPF231231 Toll-like receptor signaling pathway 104

Pathway sgRNA質粒文庫交付內容

1、混合池(成品,也可定制化)

文庫所有基因的sgRNA于1管中混合,可直接用于轉染或慢病毒包裝(圖1)。

圖1

交付內容:

  • ? 200μg液態質粒
  • ? CoA文件

2、陣列形式(定制化)

以一孔對應一靶標基因的陣列板形式提供,實現對靶標基因的靈活運用(圖2)。

圖2

交付內容:

  • ? 陣列板式液態質粒
  • ? 基因陣列排布信息
  • ? CoA文件